BacPAK™ Baculovirus Expression System
Complete kit for expressing recombinant proteins at high levels. The BacPAK System uses the baculovirus Autographa californica nuclear polyhedrosis virus (AcMNPV) to produce target proteins in insect cells. More than 90% of the viruses produced by the transfected cells carry the target protein. The expressed recombinant protein is similar in structure, biological activity, and immunological reactivity to the naturally occurring protein because insect host cells provide post-translational processing similar to that of mammalian cells.
BacPAK™6 DNA (Bsu36 I digest)
Baculovirus DNA specially designed and prepared to give a high proportion of recombinant viral expression vectors. The Bsu36 I-digested BacPAK6 DNA is ready-to-use and is sufficient for 5 transfections. The transfection reagent Bacfectin is also provided.
X-GLUC
Substrate for β-glucuronidase, an acid hydrolase encoded by the gusA gene. When used in histochemical assay methods, X-GLUC will give a blue precipitate in the presence of β-glucuronidase.
Overview
- Yields up to 1 g of protein per liter of culture
- Recombinant efficiency >90%
- Proper protein folding
- Expression of biologically active proteins
- Eukaryotic posttranslational modification
Applications
- Recombinant protein expression in insect host cells
