The B/B Homodimerizer is a membrane-permeant ligand that causes homodimerization of proteins fused to dimerization domain B (DmrB)—provided in the iDimerize Inducible Homodimer System (Cat. No. 635068). NOTE: The B/B Homodimerizer is identical to the AP20187 ligand previously supplied in the ARGENT Regulated Homodimerization Kit from ARIAD Pharmaceuticals, Inc.
B/B Washout Ligand is a membrane-permeant ligand that dissociates protein interactions induced by the B/B Homodimerizer, which is provided in each of the iDimerize Inducible Homodimer Systems (Cat. Nos. 635068, 635072, 635084, and 635086). The B/B Washout Ligand dissociates these interactions with a T1/2 of ~10 minutes after adding it to target cells treated with B/B Homodimerizer.
Overview
- Previously available from ARIAD as the ARGENT Regulated Homodimerization Kit
- Rapid chemical induction of cell signaling pathways
- Activate any pathway or cellular event controlled by homo-oligomers (e.g., dimers, trimers, or tetramers)
- Established tool used by over 2,000 research groups, with over 400 scientific publications to date
- B/B Homodimerizer is identical to the AP20187 ligand
- B/B Washout Ligand reverses protein oligomers that were induced by the B/B Homodimerizer
- Plasmid and lentiviral (Lenti-X) vector formats are available
Applications
- Induce any event or pathway that is controlled or activated by protein-protein interactions
- Activate signaling pathways in real time
- Study transmembrane receptors
- Recruit signaling proteins to specific subcellular locations (e.g., the plasma membrane)
- Inducible homodimer technology has been used for functional studies of:
- Receptor and non-receptor tyrosine kinases
- Receptor and non-receptor serine/threonine kinases
- Non-kinase receptors
- Signaling proteases
- Adaptor proteins
- Cell adhesion and rolling
- DNA looping
- Activation of recombinase
- RNA splicing
- Protein splicing
- Glycosylation
