The PCR Decontamination Kit removes contaminating DNA in PCR master mixes, without reduction of PCR sensitivity.
- The double-strand specific property of the dsDNase allows decontamination with primers and probe present.
- Efficient for end-point PCR and probe-based qPCR.
- Contaminating bacterial DNA is reduced to levels below detection limit.
- Fast and easy protocol.
Decontamination of master mixes without reduction of sensitivity has always been a challenge. Especially when minor amounts of DNA are targeted, contaminating DNA is a major problem. Any loss of sensitivity in the qPCR assay caused by the decontamination protocol is unacceptable.
Kit Contents
- DTT (Inactivation Aid)
- dsDNase (100 reactions)
Storage Conditions: Store at -20˚C.
Sample Material:The kit can be used to reduce contaminating DNA in both ordinary PCRs and probe based qPCR mixes. Also efficient for some SYBR based qPCR mixes.
Quality Control: The kit is tested for absence of RNase.
