ApopLadder Ex Kit

Additional information

Brand

Size

24 Rxns

The ApopLadder Ex Kit selectively extracts the small, fragmented DNA from apoptotic cells and minimizes chromatin contamination. This method supports highly sensitive detection and quantitation of fragmented DNA. Detection of fragmented DNA following isolation can be observed by gel electrophoresis and can be quantitated by fluorescent staining with intercalating green dye. This kit contains the necessary reagents for cell lysis, enzymatic degradation of protein and RNA, precipitation of the DNA fragments, and a 6X loading dye for gel electrophoresis.

Overview

  • Sensitive, specific, and rapid detection of fragmented DNA

Kit components

  • Lysis Buffer (4 × 1.2 ml)
  • 10 SDS (0.48 ml)
  • Enzyme A Solution (0.48 ml)
  • Enzyme B Solution (0.48 ml)
  • Precipitant (3 × 1.04 ml)
  • 6X Loading Dye (0.48 ml)