AfaI (RsaI)

Brand	Takara Bio

AfaI (RsaI) restriction site:

GT | AC
CA | TG

Supplied buffer: T+BSA

Effect of DNA methylation: enzyme activity is affected by CG methylase

Reaction temperature: 37°C

Source: Acidiphilium facilis
Concentration: 4–12 units/µl
Substrate for unit definition: lambda DNA
Each lot undergoes stringent quality testing including overdigestion, genome DNA analysis, ligation-recutting and pKF3 cloning