The Adeno-X qPCR Titration Kit is designed to be used with real-time PCR instruments to rapidly titrate adenoviral stocks from all Ad5-based adenoviral vectors. The protocol combines qPCR with green intercalating dye chemistry, allowing you to determine the viral genome copy number in adenoviral preparations (i.e., crude lysates or purified stocks) from a calibrated DNA standard curve. The kit includes a viral nucleic acid purification kit, a calibrated Adeno-X DNA Control Template, primers, and all the reagents required to conduct 200 TB Green-based qPCR reactions with either the DNA Control Template or purified viral DNA.
Overview
- Obtain a functional titer in 4 hours using either the Adeno-X Rapid Titer Kit (hexon detection) or Adeno-X qPCR Titration Kit (genome detection)
- More precise and less time-consuming than a plaque or end-point dilution assay
