Adenoviral System 3 (Universal)

The universal Adeno-X adenoviral expression systems contain vectors that lack a promoter and polyA signal in the cloning site

The universal Adeno-X adenoviral expression systems contain vectors that lack a promoter and polyA signal in the cloning site. You can either clone an expression cassette from a pre-existing construct into the vector (Panel A) or create a new one using multiple fragment In-Fusion HD Cloning (Panel B).

Adeno-X adenoviral system 3 is available in seven formats, including the most advanced tetracycline-inducible expression system, constitutive expression systems with or without fluorescent reporters, and universal systems that allow you to clone and express any entire expression cassette of your choice

Adeno-X Adenoviral System 3 is available in seven formats, including the most advanced tetracycline inducible expression system, constitutive expression systems with or without fluorescent reporters, and universal systems that allow you to clone and express any entire expression cassette of your choice.

Constructing recombinant adenovirus with In-Fusion Cloning technology

Constructing recombinant adenovirus with In-Fusion Cloning technology. DNA sequences can be rapidly transferred as PCR products to any pAdenoX vector using the In-Fusion cloning method. In this example, your gene of interest is amplified with 15 bp extensions that are homologous to the ends of the linearized adenoviral vector. The PCR product is then purified and mixed with the linearized adenoviral vector of choice in the In-Fusion reaction. Following the reaction, a portion of the mixture is transformed into E. coli (Stellar Competent Cells) and screened. Once a PCR-positive clone is identified, the recombinant pAdenoX vector is amplified, purified, and subsequently linearized with the restriction enzyme PacI, then transfected into Adeno-X 293 cells for viral rescue and amplification. Adeno-X GoStix can be used to determine the status of adenovirus rescue.