A set of three vectors encoding green fluorescent protein from Aequorea coerulescens. pAcGFP1 is a prokaryotic expression vector that also serves as a convenient source of fluorescent protein cDNA. In this vector, the full-length human-codon-optimized cDNA is flanked by distinct MCSs to facilitate excision from the vector and insertion into other eukaryotic or prokaryotic expression constructs. The other vectors, pAcGFP1-C1 and pAcGFP1-N1, are designed for studies in mammalian systems. These vectors allow expression of a protein of interest as a C- or N-terminal fusion, respectively. The fusion vectors can also be used as cotransfection markers since the unmodified vectors will express fluorescent protein. All of the fluorescent protein coding sequences in these constructs have been human-codon-optimized for efficient expression in mammalian cells.
