The AAVpro Tet-One Inducible Expression System is a tetracycline-inducible gene expression system that allows you to produce high titers of recombinant, adeno-associated virus (AAV) for establishing a tightly inducible expression system for your gene of interest in mammalian cells. The all-in-one pAAV-TetOne vector expresses the Tet-On 3G transactivator from the constitutive human PGK promoter, and your gene of interest from the PTRE3GS promoter. In the presence of doxycycline (Dox), the Tet-On 3G transactivator specifically binds and activates high-level transcription from the inducible promoter that controls expression of your gene. There is no selection marker on this vector for mammalian cells. The kit also includes the plasmids for preparation of high-titer AAV particles without the use of a helper virus, and AAV Extraction Solution for isolation of pure AAV particles.
Overview
- All-in-one vector design provides all the power of Tet-On 3G technology in a single vector, making inducible expression as easy as constitutive expression
- Low background and high maximum induced expression
- High sensitivity to doxycycline: near maximum expression at 10 ng/ml doxycycline
- Inducible expression in a broad range of tissues, because the Tet-On 3G transactivator is expressed from the human PGK promoter
- High-titer lentivirus packaging system provided with Lenti-X Tet-One systems
- Retro-X systems incude our Retro-X Universal Packaging System
- The AAVpro system uses a helper-free packaging system and includes extraction reagents for efficient isolation of AAV particles
More Information
Applications
- Tightly controlled inducible expression in mammalian cells
