Efficient concentration of AAV particles from cell culture supernatant
AAVpro Concentrator provides a simple, highly efficient alternative to ultracentrifugation for concentrating AAV particles of any serotype from cell culture supernatant. In contrast with other concentration methods that involve only precipitation of AAV particles, AAVpro Concentrator incorporates filtration steps to yield AAV preps of higher purity. The scalable mix-and-spin protocol can accommodate a range of input volumes and requires less than one hour of hands-on time. The AAVpro Concentrator reagent is provided as a 4X concentrate, which can be applied in proportion to the amount of cell culture supernatant being processed. The kit includes reagents and filtration columns for processing up to 150 ml of culture supernatant for two samples.
Overview
- Efficient concentration of AAV particles from cell culture supernatant
- Filtration steps included for removal of impurities
- Simple mix-and-spin protocol requires less than one hour of hands-on time
- Scalable method enables processing of various input volumes
- No ultracentrifugation required
- Compatible with any AAV serotype
- Can be used to process culture medium with or without serum
Applications
- Concentration of AAV from cell culture supernatant
- Transfer of AAV particles from one buffer to another
- Rescue of low-titer supernatants
- In vitro or in vivo transduction using AAV vectors
