The ICELL8 Single-Cell System is an advanced, integrated automation platform that combines the power of imaging and dispensing with the isolation of single cells in 5,184-nanowell chips. This open-platform, high-throughput system is prevalidated for use with several NGS applications, and also provides the flexibility to enable users to develop applications of their choice.
Overview
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- End-to-end automation for dispensing and isolating single cells in 5,184-nanowell chips
- Large-bore nozzle for unbiased cell size isolation
- Imaging to differentiate single from multiple cells and perform live/dead analysis with fluorescence
- Open platform ready for use with several SMARTer NGS applications or custom applications
Applications
- SMART-Seq full-length transcriptome analysis
- Differential expression by 3′ end counting
- TCR profiling and 5′ end differential expression
- ATAC-seq
- Custom applications created by you to meet your experimental needs
ICELL8 Features and Benefits
As the use of NGS advances in clinical and applied spaces, the parallel processing of large numbers of single cells will be crucial. With the increasing demand for single-cell NGS analyses, several platforms have emerged based on microfluidics, such as droplet-based and multi-sample nanodispensing technologies. The ICELL8 systems enable high-throughput processing of hundreds of single cells in a heterogeneous population without the common microfluidic cell-size constraints or the imaging limitations of droplet-based systems, thereby reducing sample-handling artifacts.
A key benefit of ICELL8 technology is the use of imaging to visualize all 5,184 nanowells to differentiate between single-cell-containing wells and multi-cell-containing wells. Furthermore, the cell samples can be stained with cellular dyes and visualized with fluorescence filters to analyze the viability of the single cells. A second key advantage of ICELL8 technology is the use of nanowell-specific barcodes to allow the tracing of sequencing data back to the individual cells that were selected for analysis. Additionally, the dispensing units of ICELL8 systems use a large-bore nozzle, providing the flexibility of working with a wide range of cell sizes—even isolated nuclei and multinucleated cells.
Images of an adult cardiomyocyte isolated and dispensed into a single nanowell on the original ICELL8 Single-Cell System. Cells were stained with Hoechst and propidium iodide. Data kindly provided by Dr. Stefan Günther, Max Planck Institute.
Features of the ICELL8 cx Single-Cell System and the original ICELL8 Single-Cell System.
