The Living Colors Anti-RCFP Polyclonal Pan Antibody was raised in a rabbit against recombinant full-length Zoanthus sp. yellow fluorescent protein (ZsYellow). This pan antibody recognizes denatured forms of AmCyan, ZsGreen, ZsYellow, DsRed2, DsRed-Express, HcRed, AsRed, as well as fusion proteins containing these RCFP variants in bacterial and mammalian cell lysates.
Overview
- Monoclonal and polyclonal antibodies to detect yellow fluorescent proteins including mBanana, ZsYellow, YFP, and EYFP
- EYFP and YFP are highly related to wild type GFP and EGFP so are detected by the same antibodies
- mBanana is detectible using one of our DsRed polyclonal antibodies
- ZsYellow is detected by our reef coral pan antibody
| Fluorescent protein | Recommended antibody | Cat. # | Proven uses* |
| mBanana | DsRed Polyclonal Antibody | 632496 | WB |
| EYFP** | A.v. Monoclonal Antibody (JL-8) | 632380, 632381 | WB, IP, IC |
| Full-Length GFP Polyclonal Antibody*** | 632592, 632593 | WB, IP, IC | |
| ZsYellow1 | Anti-RCFP Polyclonal Pan Antibody | 632475 | WB, IL |
*WB = Western Blot, IP = immunoprecipitation, IC = immunocytochemistry, IL = immunolabeling
**Not sold by Takara Bio.
***EYFP is highly related to EGFP so is detected by the same antibodies.
Applications
- Western blot
- Immunoprecipitation
- Immunocytochemistry
