pEF1α-mCherry-N1 is a mammalian expression vector that constitutively expresses a protein of interest fused to the N-terminus of the red fluorescent protein mCherry, even after stable integration of the vector into the host cell genome. Stable, constitutive expression of the fusion protein is driven by the human elongation factor 1 alpha (EF1α) promoter, allowing the monitoring of a variety of cellular processes (such as differentiation in primary or stem cells) without the transgene silencing associated with CMV promoters. The unmodified vector can be used to express mCherry in mammalian cells.
Overview
- Monoclonal and polyclonal antibodies to detect yellow fluorescent proteins including mBanana, ZsYellow, YFP, and EYFP
- EYFP and YFP are highly related to wild type GFP and EGFP so are detected by the same antibodies
- mBanana is detectible using one of our DsRed polyclonal antibodies
- ZsYellow is detected by our reef coral pan antibody
| Fluorescent protein | Recommended antibody | Cat. # | Proven uses* |
| mBanana | DsRed Polyclonal Antibody | 632496 | WB |
| EYFP** | A.v. Monoclonal Antibody (JL-8) | 632380, 632381 | WB, IP, IC |
| Full-Length GFP Polyclonal Antibody*** | 632592, 632593 | WB, IP, IC | |
| ZsYellow1 | Anti-RCFP Polyclonal Pan Antibody | 632475 | WB, IL |
*WB = Western Blot, IP = immunoprecipitation, IC = immunocytochemistry, IL = immunolabeling
**Not sold by Takara Bio.
***EYFP is highly related to EGFP so is detected by the same antibodies.
Applications
- Western blot
- Immunoprecipitation
- Immunocytochemistry
