The Vectalys Constitutive Lentiviral Reporter Vectors are prepackaged lentiviral particles expressing fluorescent protein genes for transducing mammalian cells. The expression of fluorescent protein genes is driven by an EF1α promoter. These VSV-G pseudotyped viruses are capable of infecting both dividing and non-dividing cells. The high purification level of these lentiviral particles allows transduction of primary cells and immortalized cell lines without inducing any toxicity to the cells, even at high multiplicity of infection (M.O.I.).
Overview
- What does 109 TU/ml mean?
- In each microliter, there will be at least 1 x 106 functional lentivirus particles.
- Each 60-µl kit is sufficient for 30 transductions (using an MOI of 10) in a 6-well plate containing 2 x 105 cells/well.
- Premium quality—produced using a multi-step purification process that results in:
- Very high titer; functional titer of >1 x 109 transduction units per ml (TU/ml).
- High purity—transduce target cells without cytotoxicity.
- >90% transduction efficiency even at very low MOI.
- Functional titer provided, not a just a particle count
- tdTomato and ZsGreen1 are exceptionally bright fluorescent proteins, 6X and 2.5X brighter than EGFP, respectively.
- Learn more about our fluorescent protein portfolio.
- These vectors have a puromycin resistance cassette and the stable clones integrating these constructs can be selected using puromycin.
Applications
- Labeling and tracking mammalian cells using bright fluorescent proteins
