PrimerArray Embryonic Stem Cell Primers

Procedural overview for stem cell gene expression analysis by real-time RT-PCR

The overall workflow for the PrimerArray sets is straightforward:

1. RNA extraction (30 min): we recommend NucleoSpin RNA II kits (Cat. # 740955.50) or RNAiso (Cat. # 9108, not available in all geographic locations)
2. Reverse transcription (20 min): we recommend PrimeScript RT Master Mix (Perfect Real Time) (Cat. # RR036A)
3. Dispense real-time PCR reaction solution (10 min): prepare a master mix of your cDNA sample and TB Green Premix Ex Taq II (Tli RNase Plus) (Cat. # RR820A) and dispense into a 96-well plate
4. Add PrimerArray primers (5 min): use 4 µl per reaction
5. Real-time PCR (1–2 hours): compatible with all commonly used real-time PCR systems
6. Data analysis (30 min): PrimerArray analysis tool makes data analysis easy

PrimerArray Analysis Tool for Embryonic Stem Cells

The PrimerArray Analysis Tool for Embryonic Stem Cells facilitates data analysis, and can be downloaded here. To access an instruction guide about how to use the tool, click on the Details down arrow icon in the product table, click on the Documents tab, and then expand the drop-down menu under User Manuals.

Pluripotency-related gene expression was compared in the human ES cell lines KhES-1 in different stages of differentiation using PrimerArray Embryonic Stem Cells (Cat. # PH016)

Pluripotency-related gene expression was compared in the human ES cell lines KhES-1 in different stages of differentiation using PrimerArray Embryonic Stem Cells (Cat. # PH016). RNA was prepared from cultured ES cells at an early passage (early), at a late passage (late), or after differentiation (diff). Expression data obtained from microarray analysis and the PrimerArray data were highly correlated, as shown in this figure.

Pluripotency-related gene expression was compared in the human ES cell lines KhES-3 in different stages of differentiation using PrimerArray Embryonic Stem Cells (Cat. # PH016)

Pluripotency-related gene expression was compared in the human ES cell lines KhES-3 in different stages of differentiation using PrimerArray Embryonic Stem Cells (Cat. # PH016). RNA was prepared from cultured ES cells at an early passage (early), at a late passage (late), or after differentiation (diff). Expression data from KhES-3 cells obtained from microarray analysis and the PrimerArray data were highly correlated, as shown in this figure.