This kit provides an easy and efficient method to rescue plasmid DNA from Saccharomyces cerevisiae. The protocol uses Zymolyase for highly efficient enzymatic disruption of yeast cell walls, followed by SDS/alkaline lysis of the resulting spheroplasts, and spin column purification of the plasmid DNA. Purified DNA can then be transformed into E. coli for propagation and purification.
Overview
- Rescue plasmids from yeast using spin columns
- Highly efficient Zymolyase is included
- Purify DNA in less time
Applications
- Plasmid DNA minipreps from yeast
- Rescue positive prey plasmids after a yeast two-hybrid or yeast one-hybrid screen
