TALON PMAC Magnetic Phospho Enrichment Kit

Only a small percentage of all cellular proteins are phosphorylated, so it is often necessary to enrich for this fraction before beginning an analysis

Only a small percentage of all cellular proteins are phosphorylated, so it is often necessary to enrich for this fraction before beginning an analysis. The TALON PMAC Magnetic Phospho Enrichment Kit can be used with any mammalian cell type or tissue sample. The yield of phosphorylated protein varies with different cell lines.

Highly effective enrichment of phosphorylated proteins using the TALON PMAC Magnetic Phospho Enrichment Kit

Highly effective enrichment of phosphorylated proteins using the TALON PMAC Magnetic Phospho Enrichment Kit. Proteins extracted from HEK 293 cells in Extraction/Loading Buffer containing protease inhibitors and a phosphatase inhibitor (10 mM sodium fluoride), were mixed with 200 ?l of a 5 suspension of Phospho Magnetic Beads at room temperature for 30 min, followed by washing and elution. The extract (Lanes 1), flowthrough (Lanes 2), and eluates (Lanes 3) were then analyzed by Western blotting using antibodies specific for phosphorylated AKT (Ser 473) in Panel A, P-AKT (Thr 308) in Panel B, P-PTEN (Ser 380) in Panel C, and P-GSK3 beta (Ser 9) in Panel D; the band at 51 kDa is due to cross-reactivity of the antibody with P-GSK3a.