Capturem high-capacity membranes
Capturem His-Tagged Purification Large Volume lets you quickly purify up to 25 mg of protein (depending on protein size and expression level) from a large volume of lysate (up to 500 ml at a time), resulting in purified protein concentrated at up to 2 mg/ml. Like our other his-tagged Capturem products, the Large Volume unit works for bacterial and mammalian expression systems with a wide range of additives.
Overview
- No-waiting workflow—5-minute, room-temperature protocol
- High purity and yield—small column bed volumes trap fewer contaminants and allow large-volume washes
- Compatible with wide range of conditions—native and denaturing conditions; with common additives (e.g., EDTA, DTT, BME, glycerol, TCEP, etc.; see compatibility table)
- Lysis buffer compatibility—xTractor Buffer; buffers from other vendors
- Different cell systems—easy purification from mammalian and bacterial cells
Applications
- Biochemical/enzymatic assays
- Pull-down or other protein interaction assays
- Confirmation screening
- Protein/ligand affinity analysis
- Crystallography or other structural analyses
- Antibody production
- Toxicology studies
- Protein transfer to live cells
