Glycoprotein Western Detection Kit

Highly sensitive and selective detection of purified glycoproteins with Clontech’s Glycoprotein Western Detection Kit

Highly sensitive and selective detection of purified glycoproteins with Clontech’s Glycoprotein Western Detection Kit. Panel A. Varying amounts of purified horseradish peroxidase (HRP), a known glycoprotein (Lanes 1–4), and soybean trypsin inhibitor (SBTI), a nonglycosylated protein (Lanes 5–8), were electrophoresed on a 4–15 SDS-PAGE gel and transferred to a nitrocellulose membrane. Glycoprotein bands were specifically detected in Lanes 1–4, but not Lanes 5–8, using the Glycoprotein Western Detection Kit. Lane M: molecular weight marker. Lane 1: 700 ng HRP. Lane 2: 350 ng HRP. Lane 3: 140 ng HRP. Lane 4: 70 ng HRP. Lane 5: 700 ng SBTI. Lane 6: 350 ng SBTI. Lane 7: 140 ng SBTI. Lane 4: 70 ng SBTI. Panel B. Varying amounts of three different purified glycoproteins [fetuin (Lanes 1–5), alpha-1 acid glycoprotein (AGP; Lanes 6–10), and horseradish peroxidase (HRP; Lanes 11 & 12)] were electrophoresed on a 4–15 SDS-PAGE gel, transferred to a nitrocellulose membrane, and detected using the Glycoprotein Western Detection Kit, according to the user manual. Lane M: molecular weight marker. Lane 1: 1 μg fetuin. Lane 2: 500 ng fetuin. Lane 3: 200 ng fetuin. Lane 4: 100 ng fetuin. Lane 5: 50 ng fetuin. Lane 6: 1 μg AGP. Lane 7: 500 ng AGP. Lane 8: 200 ng AGP. Lane 9: 100 ng AGP. Lane 10: 50 ng AGP. Lane 11: 350 ng HRP. Lane 12: 140 ng HRP.