The Human Cell-Free Protein Expression System from Takara Bio is easy to use. The single-tube reaction is easily assembled and protein synthesis is complete in as little as 1 hr at 32°C. The Human Cell-Free Protein Expression System provides high yield (e.g., 50 μg/ml of human eIF4G) of functional protein, including proteins requiring modifications such as glycosylation, phosphorylation, or fatty acylation and even with large proteins (over 150 kDa). Genes of interest may be cloned rapidly into pT7-IRES vectors using In-Fusion cloning technology. After expression, proteins with N-terminal or C-terminal His tag or N-terminal Myc tag can be generated, depending on choice of vector.
Overview
- Easy-to-use system allows generation of protein in as little as 1 hour in a single-tube reaction
- Provides higher yield and greater consistency of functional protein compared to rabbit reticulocyte in vitro translation systems
- Works well with challenging applications such as large proteins (over 150 kDa)
- Amenable to high-throughput screening; bulk sizes are available
Applications
- Expression of toxic proteins that are lethal for in vivo expression systems
- Quick analysis of protein functionality
- Rapid analysis of protein mutations or truncations
- Expression of proteins that are easily degraded or insoluble in conventional in vivo expression systems such as E. coli
- High-throughput proteomic studies
