Overview
Brevibacillus Competent Cells are host cells used for transformation with Brevibacillus expression vectors such as pNY326 DNA (Cat. #HB111), pNCMO2 DNA (Cat. #HB112), and pNC-HisT DNA (Cat. #HB121). Brevibacillus Competent Cells are prepared for transformation using the New Tris-PEG (NTP) method for chemical transformation of B. choshinensis strain SP3, which can provide a transformation efficiency that is approximately equal to that obtained with electroporation. The NTP method also enables direct transformation of a DNA ligation solution without DNA purification.
- Efficient production of secreted or intracellular recombinant target proteins
- Negligible amounts of extracellular protease—products remain intact in culture medium
- Unlike E. coli, no endotoxins
- Proteins are produced in active form
- Easy to culture, handle, and sterilize
