SpeedSTAR HS DNA Polymerase

Brand	Takara Bio

SpeedSTAR HS DNA Polymerase is optimized for hot-start, fast PCR, with possible extension time as fast as 10 sec/kb (compared to 60 sec/kb with general enzymes), and amplicon size up to 20 kb. SpeedSTAR reactions can be performed using standard PCR instrumentation.

Overview

Optimized for fast PCR: amplify a 2-kb fragment in as little as 30 minutes using a standard PCR machine
Excellent efficiency: robust performance, comparable to high-yield polymerases
Optimized buffers allow amplification of fragments up to 20 kb

Applications

Fast PCR: reactions can be completed in two-thirds less time
Amplification of large DNA fragments

PCR products

The majority of PCR products generated with SpeedSTAR DNA Polymerase will have 3′-A overhangs; therefore, amplification products can be directly cloned into T-vectors. It is also possible to clone amplification products into blunt-ended vectors following blunting and phosphorylation of product ends.

Amplification of an 8.5 kb Human Genomic DNA Fragment using a Standard High Yield Polymerase and SpeedSTAR HS DNA Polymerase

Amplification of an 8.5 kb Human Genomic DNA Fragment using a Standard High Yield Polymerase and SpeedSTAR HS DNA Polymerase.

Amplification of Human Genomic DNA Targets of Varying Sizes using SpeedSTAR High speed amplification is especially valuable when amplifying large-size targets

Amplification of Human Genomic DNA Targets of Varying Sizes using SpeedSTAR High speed amplification is especially valuable when amplifying large-size targets. This data shows excellent specificity and yield.

Time Comparison of SpeedSTAR and Standard High Efficiency Enzyme Reaction Times on E. coli Fragments of Varying Sizes (2-step refers to PCR cycling conditions)

Time Comparison of SpeedSTAR and Standard High Efficiency Enzyme Reaction Times on E. coli Fragments of Varying Sizes (2-step refers to PCR cycling conditions).

Time Comparison of SpeedSTAR and Standard High Efficiency Enzyme Reaction Times on Large Size Human Genomic Targets (2-step refers to PCR cycler conditions)

Time Comparison of SpeedSTAR and Standard High Efficiency Enzyme Reaction Times on Large Size Human Genomic Targets (2-step refers to PCR cycler conditions).

SpeedSTAR HS DNA Polymerase

Additional information