Applications
- Small and large RNA isolation from cultured / bacterial / yeast cells, human / animal / plant tissue, reaction mixtures
Features
- Excellent RNA recovery and purity by chaotropic salt lysis without phenol / chloroform (patent pending)
- Additional isolation of total protein fraction ready to use for SDS-PAGE and Western blot analysis
- Separation of small and large RNA possible
Specifications
- Technology: Silica membrane technology
- Sample material: Cells (< 10⁷), human / animal tissue (< 30 mg), plant tissue (< 50 mg), reaction mixtures (< 150 μL)
- Fragment size: ≥ 18 nt
- Typical yield: HeLa cells (10⁷): 10 μg small RNA, 95 μg large RNA
- Elution volume: 30–100 μL
- Binding capacity: 200 μg
- Processing time: < 45 min/6 preps total RNA)
