DNA SMART ChIP-Seq Kit — Illumina® sequencing library preparation from low-input DNA templates
The DNA SMART ChIP-Seq Kit adds Illumina-specific sequencing adapters to the ends of DNA fragments in a ligation-independent manner. Generating libraries for ChIP sequencing (ChIP-seq) can be difficult due to the small amounts of DNA recovered from ChIP experiments, particularly when using antibodies against low-abundance transcription factors. The DNA SMART ChIP-Seq Kit uses a novel version of SMART template-switching technology—adapted for DNA templates—to generate next-generation sequencing (NGS) libraries for Illumina platforms from 100 pg–10 ng of input DNA. Either ssDNA or double-stranded DNA (dsDNA) from ChIP experiments can be used directly with this kit, making it compatible with DNA recovered with the ChIP Elute Kit or by other methods. This highly sensitive kit generates robust sequencing libraries from low-input DNA samples, with minimal handling, in about four hours.
Overview
- Fast (one hour) cross-link reversal and elution of ChIP DNA
- Ligation-independent adapter addition for ChIP-seq library preparation
- Flexible DNA inputs: 100 pg–10 ng of either ssDNA or dsDNA
- Generate Illumina-ready sequencing libraries in around 4 hours
There are three versions of this kit available:
- The DNA SMART ChIP-Seq Kit – 12 contains the reagents to generate up to 12 indexed libraries using a single forward indexing primer and 12 reverse indexing primers
- The DNA SMART ChIP-Seq Kit – 48 A can generate up to 48 indexed libraries using 4 forward indexing primers and 12 reverse indexing primers
- The DNA SMART ChIP-Seq Kit – 48 B can generate up to 48 indexed libraries using 4 forward indexing primers and 12 reverse indexing primers
Note: DNA SMART ChIP-Seq Kit – 48 A and DNA SMART ChIP-Seq Kit – 48 B use different sets of forward primers with different index sequences
Applications
- Sequencing library preparation for ChIP-seq from low-input, fragmented DNA
- Illumina-specific NGS sequencing library production
