pHcRed1-Nuc Vector encodes a far-red fluorescent protein HcRed1 fused with three copies of the nuclear localization signal (NLS) of the SV40 T-antigen. The NLS sequences are fused to the 3′-end of HcRed1. HcRed1 was generated by mutagenesis of a non-fluorescent chromoprotein from the reef coral Heteractis crispa. The coding sequence for HcRed1 is human codon-optimized for higher expression in mammalian cells. pHcRed1-Nuc is designed for visualizing the nucleus in living or fixed cells by fluorescence microscopy.
