The mammalian CMV promoter in pCMV-E2-Crimson allows the constitutive expression of the far-red Living Colors fluorescent protein E2-Crimson, driven by the constitutive immediate early promoter of cytomegalovirus (CMV). pCMV-E2-Crimson does not contain an MCS. It can be used for labeling purposes or as a cotransfection marker to determine transfection efficiency. If required, stable transfectants can be selected using G418.
Overview
- Far-red fluorescent protein
- E2-Crimson fluorescent protein excitation and emission maxima: 611 and 646 nm, respectively
- Several of our red fluorescent protein antibodies strongly detect E2-Crimson
Applications
- Applications involving sensitive cells, such as primary cells and stem cells
- Fusions
- Protein localization studies
- General reporter for mammalian cells
- Monitoring transfection efficiencies
- In vivo imaging
