pEF1α-DsRed-Monomer-N1 is a mammalian expression vector that constitutively expresses a protein of interest fused to the N-terminus of the red fluorescent protein DsRed-Monomer, even after stable integration of the vector into the host cell genome. Stable, constitutive expression of the fusion protein is driven by the human elongation factor 1 alpha (EF1α) promoter, allowing the monitoring of a variety of cellular processes (such as differentiation in primary or stem cells) without the transgene silencing associated with CMV promoters. The unmodified vector can be used to express DsRed-Monomer in mammalian cells.
