pDsRed2-C1 encodes DsRed2, a variant of the Discosoma sp. red fluorescent protein (DsRed) that has been engineered for faster maturation and lower, non-specific aggregation. DsRed2 contains six amino acid substitutions that accelerate chromophore formation and decrease protein aggregation. In addition, the coding sequence for DsRed2 is human codon-optimized for high expression in mammalian cells. pDsRed2-C1 allows genes cloned into the multiple cloning site (MCS) downstream of the DsRed2 coding sequence to be expressed as fusions to the C-terminus of DsRed2 in mammalian cells.
