pDsRed2-ER is a mammalian expression vector designed to label the endoplasmic reticulum in living cells. The vector encodes a fusion consisting of Discosoma sp. red fluorescent protein (DsRed2); the endoplasmic reticulum (ER) targeting sequence of calreticulin, fused to the 5′ end of DsRed2; and the ER retention sequence, KDEL, fused to the 3′ end of DsRed2. DsRed2 is a human codon-optimized variant of wild-type DsRed that has been engineered for faster maturation and lower non-specific aggregation.
pDsRed2-ER can be introduced into mammalian cells using any standard transfection method. If required, stable transformants can be selected using G418.
