ptdTomato encodes a tandem sequence of the dimeric red fluorescent protein, Tomato. In this vector, the tdTomato coding sequence is flanked by unique restriction sites at the 5′ and 3′ ends, making it easy to excise the sequence for transfer into another vector. The ptdTomato vector contains a lac promoter sequence, and therefore can be used to express tdTomato in E. coli. It is primarily intended to serve as a source of tdTomato cDNA.
tdTomato, but not GFP, can be detected in the SCID mouse cadaver phantom model. False-color overlay images (regions of interest encircled) indicate that the imaging system could detect tdTomato fluorescence in the cadaver model, but not GFP fluorescence. Panel A. Implanted tube with 100 x 106 MDA-MB-231-tdTomato-expressing cells, imaged with the DsRed filter set. Exposure time: 1 sec. Panel B. Implanted tube with 100 x 106 MDA-MB-231-GFP-expressing cells, imaged with the GFP filter set. Exposure time: 1 sec.
Basic fluorescent protein vector map. Use this bacterial expression vector as a source of the fluorescent protein gene. Note: There is a stop codon at the 5′ end of the 3′ MCS. The 3′ MCS should not be used for cloning.
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