pZsGreen1-C1 is a human codon-optimized expression vector that encodes a variant of the Zoanthus sp. green fluorescent protein, ZsGreen1, which has been engineered for brighter fluorescence and higher expression in mammalian cells. pZsGreen1-C1 allows genes cloned into the multiple cloning site (MCS) downstream of the ZsGreen1 coding sequence to be expressed as fusions to the C-terminus of the fluorescent protein. The unmodified vector will express ZsGreen1 in mammalian cells.
Schematic for fluorescent fusion protein vectors. Panel A. N-terminal fluorescent protein (FP) vector. Panel B. C-terminal fluorescent protein vector.
Fluorescent whole-body image of a mouse with bilateral solid tumors derived from estrogen receptor-positive MCF7 human breast cancer cells. Note: the presence of ZsGreen-positive cells in tumors, lymphatic vessels (LV), and inguinal and axillary lymph nodes (LN).
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