The pLVX-AcGFP1-C1 vector is an HIV-1 based lentiviral expression vector that can produce recombinant lentivirus that efficiently transduces both dividing and non-dividing mammalian cells. This vector expresses a variant of the green fluorescent protein (GFP) from Aequorea coerulescens. pLVX-AcGFP1-C1 allows cloning of a cDNA into the multiple cloning site (MCS) downstream of the coding sequence for AcGFP1. The cDNA is subsequently expressed as a fusion to the C-terminus of the AcGFP1 protein. Thus,
this vector allows the expression of fluorescent fusion proteins in difficult-to-transfect cells. It also contains a puromycin selection marker for selection of stable transductants. The unmodified vector will express AcGFP1 protein and may be used to produce marker virus to optimize infection protocols.
