The Retro-X Tet-One Inducible Expression System is a tetracycline-inducible retroviral gene expression system that allows you to produce high titers of recombinant retroviruses for the purpose of establishing a tightly inducible expression system for your gene of interest in a wide variety of dividing mammalian cells. The all-in-one pRetroX-TetOne Vector expresses the Tet-On 3G transactivator from the constitutive human PGK promoter in the forward orientation, and your gene of interest from the PTRE3GS promoter in the reverse orientation. In the presence of doxycycline (Dox), the Tet-On 3G transactivator specifically binds and activates high-level transcription from the inducible promoter that controls expression of your gene. This vector does not contain a selection marker. The kit also includes our Retro-X Universal Packaging System, our premium retroviral packaging system featuring the GP2-293 packaging cell line. All four commonly used envelopes are supplied on separate vectors, including VSV-G, eco, ampho and 10A1, to allow you to choose the tropism that is most appropriate for your target cells.
Overview
- Single-vector format for a simple protocol
- Outstanding induction control and extremely low basal expression for a broad range of dividing cell types
- TRE3G promoter is optimized for very tight transcriptional control
- Supplied with our best-performing retroviral packaging system (Retro-X Universal)
- Two-vector Tet-On 3G format also available
Applications
- Establish a tetracycline-inducible gene expression system in a wide variety of dividing cells using retrovirus
