XbaI

Brand	Takara Bio

XbaI restriction site:

T | CTAG A
A GATC | T

Supplied buffer: M+BSA

Effect of DNA methylation: enzyme activity is affected by dam methylase

Reaction temperature: 37°C

Source: Xanthomonas badrii
Concentration: 8–20 units/µl [High concentration: 30–60 units/µl]
Substrate for unit definition: N6-methyladenine free lambda DNA
Genomic DNA analysis: Escherichia coli genomic DNA
Star activity: cleavage at alternative site(s) occurs in the presence of DMSO or high concentrations of glycerol
Each lot undergoes stringent quality testing including overdigestion, genome DNA analysis, ligation-recutting and pKF3 cloning