SpeI

Brand	Takara Bio

SpeI restriction site:

A | CTAG T
T GATC | A

Supplied buffer: M

Reaction temperature: 37°C

Source: Sphaerotilus natans
Concentration: 4–12 units/µl
Substrate for unit definition: pASf2-XbaI DNA
Genome DNA analysis: Escherichia coli genomic DNA
Star activity: cleavage at alternative site(s) occurs in the presence of DMSO or at low ionic strength
Each lot undergoes stringent quality testing including overdigestion, genome DNA analysis, ligation-recutting and pKF3 cloning