ScaI

Brand	Takara Bio

ScaI restriction site:

AGT | ACT
TCA | TGA

Supplied buffer: H

Reaction temperature: 37°C

Source: Streptomyces caespitosus
Concentration: 4–12 units/µl
Substrate for unit definition: lambda DNA
Star activity: unrelated sites may often be cut in the presence of Mn2+, and at alkaline pH or low ionic strength
Do not dilute. This enzyme is unstable at low concentrations. Addition of BSA to a final concentration of 0.01 in reaction mixture increases the relative activity up to 120 at 30°C and up to 240 at 25°C, but does not increase at 37°C.
Each lot undergoes stringent quality testing including overdigestion, genome DNA analysis, ligation-recutting and pKF3 cloning