MseI (RspRSII)

Brand	Takara Bio
Size	500 Units

Brand

Size

500 Units

MseI (RspRSII) restriction site:

T | TA A
A AT | T

Supplied buffer: T+BSA

Effect of DNA methylation: not sensitive to DNA methylation

Reaction temperature: 60°C

Source: Escherichia coli carrying a plasmid encoding the RspRSII gene
Concentration: 10 units/µl
Substrate for unit definition: lambda DNA
Star activity: cleavage at alternative site(s) occurs in the presence of high concentrations of glycerol
Inactivation: Residual activity observed after EtOH precipitation or incubation at 70°C for 15 minutes. No residual activity observed after phenol treatment.
MseI, an isoschizomer of RspRSII, is a restriction enzyme that cleaves within the recognition sequence 5′-TTAA-3′. MseI is exceptionally stable and has an optimal reaction temperature of 60°C. It can be used to pretreat (fragment) genomic DNA prior to enrichment of methylated DNA using the EpiXplore Methylated DNA Enrichment Kit.
Each lot undergoes stringent quality testing including overdigestion, genome DNA analysis, ligation-recutting and pKF3 cloning