McrBC

Brand	Takara Bio
Size	200 Units

Brand

Size

200 Units

McrBC restriction site:

5′ …(A/G)mC(Nn)(A/G)mC…3′
mC : 5-hydroxymethylcytosine, 5-methylcytosine, 4-methylcytosine, n=40-2,000

Supplied buffer: McrBC
Reaction temperature: 37°C

Source: recombinant proteins expressed in E. coli
Concentration: 1 unit /µl
Substrate for unit definition: pBR322
McrBC specifically cleaves DNA containing methyl cytosine (mC : 5-hydroxymethylcytosine, 5-methylcytosine, 4-methylcytosine) preceded by a purine nucleotide (Pu : A or G). DNA cleavage by McrBC requires at least two PumC sites within a distance of 40–2,000 bp. McrBC requires GTP for cleavage.
Each lot undergoes stringent quality testing including overdigestion, genome DNA analysis, ligation-recutting and pKF3 cloning