FokI

Brand	Takara Bio

FokI restriction site:

GGATG(N)9 |
CCTAC(N)13 |

Supplied buffer: M + BSA

Effect of DNA methylation: enzyme activity is not affected by CG methylase

Reaction temperature: 37°C

Source: Escherichia coli UT 481 carrying the plasmid encoding the fokI gene
Concentration: 4–12 units/µl
Substrate for unit definition: lambda DNA
Each lot undergoes stringent quality testing including overdigestion, genome DNA analysis, ligation-recutting and pKF3 cloning