EcoT22I (AvaIII)

Brand	Takara Bio

EcoT22I (AvaIII) restriction site:

A TGCA | T
T | ACGT A

Supplied buffer: H

Reaction temperature: 37°C

Source: Escherichia coli WA 921
Concentration: 4–12 units/µl
Substrate for unit definition: lambda DNA
Star activity: unrelated site may often be cut in the presence of 2-mercaptoethanol, and at low ionic strength
Each lot undergoes stringent quality testing including overdigestion, genome DNA analysis, ligation-recutting and pKF3 cloning