EcoO65I (BstEII, BstPI)

Brand	Takara Bio

EcoO65I (BstEII, BstPI) restriction site:

G | GTNACC
CCANTG | G

Supplied buffer: H+BSA

Effect of DNA methylation: enzyme activity is not affected by CG methylase

Reaction temperature: 37°C

Source: Escherichia coli K11a
Concentration: 4–12 units/µl
Substrate for unit definition: lambda DNA
Star activity: unrelated site may often be cut in the presence of DMSO, and at low ionic strength
Each lot undergoes stringent quality testing including overdigestion, genome DNA analysis, ligation-recutting and pKF3 cloning