BglII

Brand	Takara Bio

BglII restriction site:

A | GATC T
T CTAG | A

Supplied buffer: H

Effect of DNA methylation: enzyme activity is not affected by dam methylase

Reaction temperature: 37°C

Source: Bacillus globibii
Concentration: 10 units/μl [High concentration: 50 units/μl]
Substrate for unit definition: lambda DNA
Star activity: unrelated site may often be cut in the presence of DMSO
Each lot undergoes stringent quality testing including overdigestion, genome DNA analysis, ligation-recutting and pKF3 cloning