ApaI

Brand	Takara Bio

ApaI restriction site:

G GGCC | C
C | CCGG G

Supplied buffer: L

Effect of DNA methylation: enzyme activity is affected by CG methylase

Reaction temperature: 37°C

Source: Acetobacter pasteurianus sub. pasteurianus
Concentration: 8–20 units/μl; 30–60 units/μl (high concentration)
Substrate for unit definition: lambda DNA
Genome DNA analysis: Staphylococcus aureus genome DNA
Definition of activity: 3 units for lambda DNA as substrate is equivalent to 1 unit for an Ad2 substrate
Each lot undergoes stringent quality testing including overdigestion, genome DNA analysis, ligation-recutting and pKF3 cloning