AflII

Brand	Takara Bio

AflII restriction site:

C | TTAA G
G AATT | C

Supplied buffer: M + BSA

Reaction temperature: 37°C

Source: Anabaena flos-aquae
Concentration: 4–12 units/µl
Substrate for unit definition: lambda DNA
Ligation-recutting test: The ligation efficiency of cohesive ended DNA fragments generated by this enzyme will be lower than expected. More efficient ligation can be achieved using blunt end ligation reaction conditions.
Each lot undergoes stringent quality testing including overdigestion, genome DNA analysis, ligation-recutting and pKF3 cloning