Shrimp alkaline phosphatase (SAP)
Shrimp alkaline phosphatase (SAP) nonspecifically catalyzes the dephosphorylation of 5′-termini DNA and RNA phosphomonoesters, but does not degrade diphosphate or triphosphate linkages. The enzyme acts on 5′ protruding, recessive, and blunt 5′-termini. Unlike Calf intestinal alkaline phosphatase, SAP is irreversibly heat inactivated at 65°C for 15 minutes, allowing for streamlining of cloning experiments.
Applications
- Dephosphorylation of DNA 5′-termini
- Reduction of vector re-ligation and background
