M-SAN High Quality for Bioprocessing is a nonspecific endonuclease that is highly active at physiological conditions. M-SAN High Quality can be used directly in expression media, which can improve efficiency and yield in various workflows.
The Main Advantages of M-SAN High Quality
- High purity (≥ 98%)
- No protease detected
- Animal origin-free production
- Endotoxin-tested
- High activity at physiological salt and pH
- Supplied with extended product documentation
Properties
- Source: Recombinantly produced in Pichia pastoris
- Size: Protein size is 24.5 kDa.
- pI: 8.62.
- Unit definition: One Unit is defined as an increase in absorbance at 260 nm of 1 A in 30 minutes at 37°C, using 50 μg/ml calf thymus DNA in a buffer consisting of 25 mM Tris-HCl pH 7.6 @ 25°C, 2.5 mM MgCl2, 150 mM NaCl.
- Activity:
- Tmp: 25–40°C, optimal 37°C
- NaCl: Working range (>20%): 50-400mM. Optimal range (>80%): 125mM–250mM
- Mg2+ (>1mM) is required for activity, optimal range Mg2+ is >4 mM
- pH: Working range (>20%): 6.5-10.0. Optimal range (>80%): 7.2–9.5
- Tolerance to typical buffer additives:
- DTT and other reducing agents may inactivate M-SAN HQ
- Urea: Not recommended
- EDTA: Not recommended
