T4 RNA Ligase

T4 RNA Ligase

T4 RNA Ligase is a ligation enzyme for joining single-stranded RNA or DNA fragments by catalyzing the formation of phosphodiester bonds between single-stranded RNA or DNA containing 5′-phosphate and 3′-hydroxyl termini. This enzyme requires ATP for activity, and its activity is highest for RNA-RNA ligation, lower for RNA-DNA ligation, and lowest for DNA-DNA ligation. 3′-end labeling is often completed using 5′-[32P]pCp.

Applications

• Ligation of single-stranded RNA or DNA
• 3′-end labeling of single stranded RNA or DNA