IkappaBalpha Dominant-Negative Vector Set

Signal transduction kits are available for in vivo studies of signal transduction—from signaling cascades to gene transcription

Signal transduction kits are available for in vivo studies of signal transduction—from signaling cascades to gene transcription.

Inhibition of NFκB activity using pCMV-IκBα and pCMVκIαBM from the IκB Dominant-Negative Vector Set

Inhibition of NFκB activity using pCMV-IκBα and pCMVκIαBM from the IκB Dominant-Negative Vector Set (Cat. # 631923). HEK 293 cells were transiently transfected with the indicated plasmids. After 16 hr, fresh medium was added to the cells along with TNF to induce the NFκB pathway. SEAP activity was assayed 6 hr later to determine NFκB activity.