Synthetic siRNA Quantitation Core Kit is designed to quantify synthetic siRNA when used in combination with realtime PCR analysis, and is used to assay the residual amount of the administered siRNA in total RNA samples extracted from cells, tissues, blood, and other specimens.
6142 (for 30 reactions)1
| Terminal Transferase Buffer | 390 µl |
| Terminal Transferase | 30 µl |
| dATP | 150 µl |
| Recombinant RNase Inhibitor | 30 µl |
| Oligo dT Primer2 | 150 µl |
| RT Buffer | 270 µl |
| RT Enzyme Mix | 30 µl |
| Universal Primer (10 µM) | 120 µl |
| EASY Dilution (for Real Time PCR) | 1 ml |
| Control siRNA (20 nM) | 10 µl |
| Control Primer (10 µM) | 20 µl |
1 Contains sufficient reagent for 30 poly dA tailing and reverse transcription reactions and sufficient Universal Primer for 120 real time PCRs.
2 Product-specific oligo dT primer
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