The Ready-To-Glow MetLuc Monoclonal Antibody recognizes Metridia luciferase expressed by mammalian cells, as well as recombinant Metridia luciferase, in amounts down to 20 ng using Western blot analysis.
Overview
- Secreted luciferase assay protocol eliminates cell lysis
- 2–4 fold higher signal than firefly or Renilla luciferase
- Single transfection allows multiple time points from same cell
- Dual, live-cell assay includes SEAP as well as secreted luciferase:
monitor two promoters, or use one reporter as a normalization control
